Fig. 4. Serum recognition of MUC1 expressing breast and prostate cancer cell lines and complement-mediated killing. (a) Elicited MUC1 anti-sera show high affinity at high dilution (1° Ab, anti-serum; blue: non-immunized mice; red: 1/500 dilution; yellow: 1/200 dilution; 2°: anti-murine IgG-AF647); (b) anti-MUC1 serum mediates effective complement-directed killing of MUC1-positive cancer cells (cells were incubated with serum (1 : 10) for 30 min, then medium was changed to 10% rabbit serum as a source of complement, followed by incubation for another 2 h. Cell viability was tested by MTT assay. Error bars: mean ± SD, n = 3).
