Fig. 5. T cell activation and cytokine profile after co-culture with VHH7 or VHH7–MUC1-pulsed naive APCs. (a) CD4 T cell cytokine profile with splenic DCs as APCs; (b) CD8 T cell cytokine profile with splenic DCs as APCs; (c) CD4 T cell cytokine profile with B cells as APCs; (d) CD8 T cell cytokine profile with B cells as APCs. DC and B cells from naïve mice and T cells from immunized mice were purified by immunomagnetic selection. DC and B-cells were pulsed with VHH7 or VHH7–MUC1(Tn) conjugates, and then co-cultured with T cells for 24 h or 48 h before flow cytometric analysis. Error bars: mean ± SD, n = 3. Brefeldin A (BFA) was added 12 h prior to analysis to ensure intracellular retention of the indicated cytokines.
