Figure 7.

Changes in deuterium incorporation resulting from the presence of AA and 15-HpETE. The crystal structure of 15-LOX with bound Ca²⁺ and C8E4 is used to depict connections between the PLAT and catalytic domains.¹⁶ Peptides that exhibited a decrease in the level of deuterium incorporation of >10% upon AA addition following incubation in D₂O for 2 min are colored blue. Peptides with no changes are colored gray, and unidentified peptides are colored tan. C8E4 is colored yellow to demonstrate the putative orientation of AA in the 15-LOX-2 active site, and Ca²⁺ is colored purple.