Table 2. Strains of polyhydrolytic aerobic haloalkaliphilic bacteria, isolated from soda solonchak soils.
Candidate new species are highlighted in bold (<97% 16S rRNA gene sequence identity). “ +” and “ −” presence or absence of the feature.
Isolate code | Source | Colony morphology | Phylogeny | |||
---|---|---|---|---|---|---|
Sample code | Mycelium | Pigment aerial/substrate | Endo-spores | Closest relative | % similarity | |
Actinobacteria | ||||||
DS1 | KUS | + | − | − | Streptomyces sodiiphilus (haloalkaliphile) | 97 |
DS7 | BS | + | Gray | − | Streptomyces sodiiphilus (haloalkaliphile) | 97 |
DS8 | BS | + | − | − | Streptomyces sodiiphilus (haloalkaliphile) | 97 |
DS9 | BS | + | Gray | − | Streptomyces alkaliphilus (haloalkaliphile) | 99 |
DS16 | KT | + | − | − | Streptomyces alkalithermotolerans (haloalkaliphile) | 98 |
DS31 | EWN | + | Gray | − | Streptomyces sp. E-070 (haloalkaliphile) | 99 |
DS32 | EWN | + | − | − | Streptomyces sp. E-070 (haloalkaliphile) | 99 |
DS34 | MLC | + | Gray | − | Streptomyces sp. YIM 80244 (haloalkaliphile) | 97 |
DS35 | MLC | + | Beige | − | Streptomyces sp. E-070 (alkaliphile) | 99 |
DS36 | KS | + | Gray | − | Streptomyces sodiiphilus YIM 80305 (haloalkaliphile) | 99 |
DS37 | KS | + | Gray | − | Streptomyces alkaliphilus (haloalkaliphile) | 99 |
DS39 | KS | + | -/brown | − | Streptomyces sp. E-070 (haloalkaliphile) | 99 |
DS42 | KS | + | Beige | − | Streptomyces alkalithermotolerans (haloalkaliphile) | 97 |
DS43 | KS | + | Beige | − | Streptomyces sodiiphilus | 99 |
DS46 | KS | + | Gray | − | Streptomyces sp. E-070 (haloalkaliphile) | 99 |
DS55 | AA | + | − | − | Streptomyces sodiiphilus (haloalkaliphile) | 97 |
DS58 | KS | + | − | − | Streptomyces sodiiphilus YIM 80305 (haloalkaliphile) | 97 |
DS59 | KS | + | − | − | Streptomyces sodiiphilus YIM 80305 (haloalkaliphile) | 97 |
DS61 | KS | + | Beige | − | Streptomyces sunnurensis | 98 |
DS65 | AA | + | Gray | − | Streptomyces alkaliphilus (haloalkaliphile) | 99 |
DS70 | AA | + | − | − | Streptomyces alkalithermophilus (alkaliphile) | 97 |
DS71 | AA | + | Gray/red | − | Streptomyces alkaliphilus (haloalkaliphile) | 99 |
DS177 | KS | − | Gray/viol | − | Streptomyces alkaliphilus (haloalkaliphile) | 99 |
DS182 | KT | + | Olive | − | Streptomyces alkaliphilus (haloalkaliphile) | 99 |
DS183 | KT | + | − | − | Streptomyces sp. E-070 (haloalkaliphile) | 97 |
DS2 | KUS | + | − | − | Nocardiopsis exhalans VTT E-063001 | 99 |
DS3 | KUS | + | − | − | Nocardiopsis sp. YIM 80251 (haloalkaliphile) | 99 |
DS4 | KUS | + | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS10 | BS | + | − | − | Nocardiopsis exhalans VTT E-063001 | 99 |
DS12 | KT | + | − | − | Nocardiopsis sp. YIM 80129 (haloalkaliphile) | 99 |
DS13 | KUS | + | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS14 | KT | + | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS15 | KT | + | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS17 | MS | + | Beige | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS18 | MS | + | Beige | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS19 | MS | + | Gray | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS21 | MS | + | Olive | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS22 | MS | + | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS23 | MS | + | Beige | − | Nocardiopsis sp. YIM 80251 (haloalkaliphile) | 99 |
DS24 | MS | + | Beige | − | Nocardiopsis sp. YIM 80251 (haloalkaliphile) | 99 |
DS25 | MS | + | Beige | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS26 | MS | + | Beige | − | Nocardiopsis sp. YIM 80133 (haloalkaliphile) | 99 |
DS27 | MS | + | Beige | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS28 | MS | + | -/brown | − | Nocardiopsis sp. YIM 80133 (haloalkaliphile) | 99 |
DS29 | MS | + | − | − | Nocardiopsis sp. YIM 80133 (haloalkaliphile) | 99 |
DS30 | MS | + | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS38 | KS | + | Beige/red | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS40 | KS | + | Beige | − | Nocardiopsis sp. YIM 80129 (haloalkaliphile) | 99 |
DS41 | KS | + | Beige | − | Nocardiopsis sp. AACh2 (haloalkaliphile) | 99 |
DS44 | KS | + | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS45 | KS | + | − | − | Nocardiopsis sp. YIM 80129 (haloalkaliphile) | 100 |
DS47 | AA | + | − | − | Nocardiopsis alba | 99 |
DS48 | AA | + | − | − | Nocardiopsis alba | 98 |
DS49 | AA | + | − | − | Nocardiopsis sinuspersici | 99 |
DS50 | AA | + | − | − | Nocardiopsis sp. YIM 80133 (haloalkaliphile) | 99 |
DS51 | AA | + | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS53 | AA | + | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS54 | AA | + | -/red | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS56 | AA | + | − | − | Nocardiopsis alba | 99 |
DS57 | KS | + | Beige | − | Nocardiopsis sp. YIM 80133 (haloalkaliphile) | 99 |
DS62 | KS | + | Olive | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS63 | AA | + | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS64 | AA | + | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS66 | AA | + | − | − | Nocardiopsis sp. YIM 80130 (haloalkaliphile) | 99 |
DS67 | AA | + | − | − | Nocardiopsis sp. AACh2 (haloalkaliphile) | 99 |
DS68 | AA | + | − | − | Nocardiopsis sp. YIM 80130 (haloalkaliphile) | 99 |
DS69 | AA | + | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS73 | KUS | + | − | − | Nocardiopsis sp. AACh2 (haloalkaliphile) | 99 |
DS74 | KUS | + | − | − | Nocardiopsis sp. AACh2 (haloalkaliphile) | 99 |
DS75 | KUS | + | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS76 | KUS | + | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS78 | KUS | + | − | − | Nocardiopsis sp. YIM 80130 (haloalkaliphile) | 99 |
DS79 | KUS | + | − | − | Nocardiopsis sp. AACh2 (haloalkaliphile) | 99 |
DS174 | KS | − | -/red | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 | |
DS175 | KS | − | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS176 | KS | − | − | − | Nocardiopsis sp. E-143 (haloalkaliphile) | 99 |
DS178 | KS | − | − | − | Nocardiopsis sp. YIM 80034 (haloalkaliphile) | 100 |
DS180 | KUS | + | Reddish | − | Nocardiopsis ganjiahuensis (haloalkaliphile) | 100 |
DS181 | KUS | + | − | − | Nocardiopsis sp. AACh2 (haloalkaliphile) | 99 |
DS20 | MS | + | − | − | Glycomycetaceae(halophiles) | 92 |
DS33 | EWN | + | Pink | − | Salinispora arenicola NPS11684 | 94 |
DS60 | KS | + | − | − | Isoptericola halotolerans | 99 |
DS82 | KT | − | Yellow | + | Isoptericola halotolerans | 99 |
DS88 | KS | − | Yellow | − | Isoptericola halotolerans | 99 |
DS91 | KT | − | Yellow | − | Isoptericola halotolerans | 99 |
DS92 | KT | − | Yellow | − | Isoptericola halotolerans | 99 |
DS97 | MS | − | Yellow | − | Isoptericola halotolerans | 99 |
DS99 | MS | − | − | − | Isoptericola halotolerans | 99 |
DS111 | MS | − | Yellow | − | Isoptericola halotolerans | 98 |
DS164 | KS | − | Yellow | − | Isoptericola halotolerans | 99 |
DS149 | MS | − | Orange | − | Nesterenkonia xinjiangensis | 100 |
DS11 | KUS | − | − | − | Nitriliruptor alkaliphilus (haloalkaliphile) | 98 |
Bacilli | ||||||
DS6 | BS | + | − | + | Bacillus horikoshii (alkaliphile) | 100 |
DS72 | KUS | − | − | + | Bacillus sp. E-141 (haloalkaliphile) | 99 |
DS81 | KT | − | − | + | Bacillus okhensis (haloalkalitolerant) | 99 |
DS83 | KT | − | − | + | Bacillus sp. ABCh1 (haloalkaliphile) | 98 |
DS84 | KT | − | Yellow | + | Bacillus cellulolyticus (alkaliphile) | 99 |
DS85 | KT | − | − | + | Bacillus cellulolyticus (alkaliphile) | 99 |
DS86 | KT | − | Cream | + | Bacillus pseudofirmus ( alkaliphile) | 100 |
DS87 | KT | − | − | + | Bacillus polygoni (haloalkaliphile) | 99 |
DS89 | KS | − | − | + | Bacillus daliensis (haloalkaliphile) | 99 |
DS90 | KT | − | − | + | Bacillus halodurans ( haloalkalitolerant) | 100 |
DS93 | KT | − | − | + | Bacillus cellulolyticus (alkaliphile) | 100 |
DS94 | KT | − | − | + | Bacillus vedderi (alkaliphile) | 98 |
DS95 | KT | − | − | + | Bacillus akibai (alkaliphile) | 98 |
DS96 | MS | − | Orange | − | Bacillus halodurans (haloalkaliphile) | 99 |
DS100 | MS | − | Orange | + | Bacillus daliensis (haloalkaliphile) | 98 |
DS101 | MS | − | − | + | Bacillus akibai (alkaliphile) | 99 |
DS102 | MS | − | − | + | Bacillus alkalisediminis (haloalkaliphile) | 98 |
DS103 | MS | − | − | + | Bacillus akibai (alkaliphile) | 99 |
DS104 | MS | − | − | + | Bacillus alkalisediminis (haloalkaliphile) | 98 |
DS105 | MS | − | − | + | Bacillus akibai (alkaliphile) | 99 |
DS106 | MS | − | − | + | Bacillus alkalisediminis (haloalkaliphile) | 98 |
DS107 | MS | − | − | + | Bacillus akibai (alkaliphile) | 99 |
DS108 | MS | − | − | + | Bacillus alkalisediminis (haloalkaliphile) | 98 |
DS109 | MS | − | − | + | Bacillus alkalisediminis (haloalkaliphile) | 98 |
DS110 | MS | − | − | + | Bacillus akibai (alkaliphile) | 99 |
DS112 | MS | − | − | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS113 | KS | − | Orange | − | Bacillus daliensis (haloalkaliphile) | 99 |
DS114 | KT | − | − | + | Bacillus bogoriensis (haloalkaliphile) | 97 |
DS116 | KT | − | − | + | Bacillus sp. Z24-11 (haloalkaliphile) | 100 |
DS118 | KT | − | − | + | Bacillus polygoni (alkaliphile) | 99 |
DS119 | KT | − | − | + | Bacillus pseudofirmus (alkaliphile) | 100 |
DS120 | KT | − | − | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS121 | KT | − | − | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS122 | KT | − | Cream | + | Bacillus pseudofirmus (alkaliphile) | 98 |
DS126 | BS | − | − | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS127 | BS | − | Orange | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS128 | BS | − | Orange | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS129 | BS | − | − | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS131 | BS | − | Orange | − | Bacillus pseudofirmus (alkaliphile) | 100 |
DS132 | KT | − | Cream | + | Bacillus polygoni (haloalkaliphile) | 99 |
DS133 | KT | − | − | + | Bacillus halodurans (haloalkaliphile) | 100 |
DS134 | KT | − | Cream | + | Bacillus clarkii (alkaliphile) | 99 |
DS135 | KT | − | − | + | Bacillus polygoni (haloalkaliphile) | 99 |
DS136 | KT | − | Cream | + | Bacillus sp. Z24-11 (haloalkaliphile) | 99 |
DS137 | KT | − | − | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS138 | KT | − | − | + | Bacillus sp. Z24-11 (haloalkaliphile) | 99 |
DS139 | KT | − | − | + | Bacillus polygoni (haloalkaliphile) | 100 |
DS140 | KT | − | − | + | Bacillus alkalisediminis (haloalkaliphile) | 99 |
DS141 | KT | − | Yellow | + | Bacillus alkalinitrilicus (haloalkaliphile) | 99 |
DS142 | KT | − | − | + | Bacillus alkalinitrilicus (haloalkaliphile) | 99 |
DS143 | KT | − | − | + | Bacillus mannanilyticus (alkaliphile) | 96 |
DS144 | MS | − | − | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS148 | MS | − | − | + | Bacillus alkalinitrilicus (haloalkaliphile) | 99 |
DS150 | MS | − | Orange | + | Bacillus daliensis (haloalkaliphile) | 98 |
DS151 | MS | − | − | + | Bacillus halodurans (haloalkaliphile) | 100 |
DS152 | MS | − | − | + | Bacillus horikoshii (alkaliphile) | 99 |
DS153 | MS | − | − | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS155 | MS | − | − | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS158 | MS | − | − | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS159 | MS | − | − | + | Bacillus akibai (alkaliphile) | 99 |
DS160 | KS | − | Yellow | + | Bacillus horikoshii (alkaliphile) | 99 |
DS161 | KS | − | − | + | Bacillus horikoshii (alkaliphile) | 99 |
DS163 | KS | − | − | + | Bacillus pseudofirmus (alkaliphile) | 100 |
DS165 | KS | − | − | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS166 | KS | − | − | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS168 | KS | − | − | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS169 | KS | − | − | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS172 | KS | − | − | + | Bacillus pseudofirmus (alkaliphile) | 99 |
DS184 | KT | − | − | + | Bacillus halodurans (haloalkaliphile) | 100 |
DS117 | KT | − | Orange | − | Anaerobacillus alkalidiazotrophicus (haloalkaliphile) | 97 |
DS123 | KT | − | − | + | Anaerobacillus alkalidiazotrophicus (haloalkaliphile) | 97 |
Gammaproteobacteria | ||||||
DS115 | KUS | − | − | − | Alkalimonas amylolytica (haloalkaliphile) | 99 |
DS125 | BS | − | − | − | Alkalimonas collagenimarina (haloalkaliphile) | 99 |
DS130 | BS | − | − | − | Alkalimonas amylolytica (haloalkaliphile) | 99 |
DS154 | MS | − | Greenish | − | Alkalimonas amylolytica (haloalkaliphile) | 99 |
DS124 | BS | − | − | − | Aliidiomarina maris | 99 |
DS145 | MS | − | − | − | Aliidiomarina soli (haloalkaliphile) | 99 |
DS146 | MS | − | − | − | Aliidiomarina soli (haloalkaliphile) | 99 |
DS156 | MS | − | − | − | Aliidiomarina soli (haloalkaliphile) | 99 |
DS157 | MS | − | − | − | Aliidiomarina soli (haloalkaliphile) | 99 |
DS167 | KS | − | − | − | Aliidiomarina soli (haloalkaliphile) | 99 |
DS179 | KS | − | − | − | Aliidiomarina soli (haloalkaliphile) | 98 |
DS162 | KS | − | Yellow | − |
Xanthomonadaceae ML-122 (haloalkaliphile) Rehaibacterium terrae |
97 95 |
DS170 | KS | − | − | − | Xanthomonadaceae ML-122 (haloalkaliphile) | 99 |
DS171 | KS | − | − | − | Lysobacterspp. | 96 |
DS173 | KS | − | Yellow | − |
Xanthomonadaceae ML-122 (haloalkaliphile) Lysobacterspp. |
99 95 |
DS147 | MS | − | − | − |
Xanthomonadaceae ML-122 (haloalkaliphile) Lysobacterspp. |
99 95 |