Table 4. Influence of pH on growth and endoglucanase activity of soda solonchak alkaliphiles: average profiles estimated from individual results for eight isolates: actinomycetes-Nocardiopsis DS50, 51; Streptomyces DS8,9; Bacillus: DS85, 100, 101, 102.
pH | % of maximum | |||
---|---|---|---|---|
Actinomycetes | Bacillus ACB | |||
Growth | Activity | Growth | Activity | |
5 | 0 | 0 | ||
6 | 20–70 | 30–70 | 0 | |
7 | 40–100 | 70–100 | 0–10 | 0–40 |
8 | 80–100 | 90–100 | 20–60 | 40–100 |
9 | 90–100 | 90–100 | 70–100 | 90–100 |
10 | 80–100 | 90–100 | 100 | 90–100 |
10.5 | 40–90 | 70–100 | 80–100 | 100 |
11 | 10–40 | 40–80 | 30–70 | 50–90 |
Notes.
Solid medium 0.6 M total Na+ buffered with: pH 5–8-0.1 M HEPES/NaCl/NaHCO3; pH 8–11-NaHCO3/Na2CO3. Substrate: 0.1% CMC + yeast extract 0.2 g/l. Growth and activity were estimated by the diameter of colony and zone of hydrolysis, respectively, after four days of plate incubation at 30 °C.