|
| ||
| Reagent | Volume (μl) | Final Concentration |
|
| ||
| 10X PCR buffer I with 15 mM MgCl2 | 2 | 1X, 1.5 mM MgCl2 |
|
| ||
| 25 mM MgCl2 | 2.8 | 3.5 mM |
|
| ||
| dNTPs (2.5 mM) | 8 | 1 mM |
|
| ||
| Random hexamer 50 μM | 1 | 2.5 μM |
|
| ||
| RNase inhibitor (40 U/μl) | 1 | 40 U |
|
| ||
| Reverse transcriptase (50 U/μl) | 1 | 50 U |
|
| ||
| Extracted RNA | 4.2 | varied |
|
| ||
| Total | 20 | |
|
| ||
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.