|
| ||
| Reagent | Volume (μl) | Final Concentration |
|
| ||
| dH2O | 7.6 | Not applicable |
|
| ||
| 25 mM MgCl2 | 2.4 | 3 mM |
|
| ||
| Primer/Probe Master Mix (see ‘Materials’) | 3 | 0.75 μM primer0.375 μM probe |
|
| ||
| 10X LightCycler® FastStart DNA Master HybProbe | 2 | 1X |
|
| ||
| cDNA from step ii | 5 | varied |
|
| ||
| Total | 20 | |
|
| ||
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