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. 2017 Sep 28;12(9):e0182707. doi: 10.1371/journal.pone.0182707

Fig 4. Effects of CA and ORXA treatment (50 μM) on mRNA levels of ORX receptors in different brain structures.

Fig 4

(A) mRNA levels of Orexin receptor 1 and 2 in different brain structures as compared to Cerebellum. (B-C) Effects of CA and ORXA treatment on mRNA levels of ORX receptor-1 (B), and ORX receptor-2 (C). Results are expressed relative to the control group. Asterisks and pound sings indicate significant differences from Sham control or CA+Saline group, respectively, as a result of LSD post-hoc tests with p levels corrected for multiple comparisons, pcor<0.0021 (see S5 Table for results of ANOVA). Probe sequences for RT-PCR are presented in S2 Table.