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. 2017 Sep 1;73(Pt 9):536–540. doi: 10.1107/S2053230X17012419

Table 1. Macromolecule-production information.

Source organism M. hassiacum DSM 44199T (Tiago et al., 2012)
DNA source pET-30a-MhGgH (Alarico et al., 2014)
Forward primer TATACGTCTCACATGCCGCACGACCCGAGTTTCAC
Reverse primer CGAATTCTTAGCCCAGCCAGTCGAGCACC
Cloning and expression vector pETM11
Expression host MhGgH, E. coli BL21 (DE3); SeMet-MhGgH, E. coli B834 (DE3)
Complete amino-acid sequence of the construct produced§ MKHHHHHHPMSDYDIPTTENLYFQGAMPHDPSFTPTQLAARAAYLLRGNDLGTMTTAAPLLYPHMWSWDAAFVAIGLAPLSVERAVVELDTLLSAQWRNGMIPHIVFANGVDGYFPGPARWATATLADNAPRNRLTSGITQPPVHAIAVQRILEHARTRGRSTRAVAEAFLDRRWGDLMRWHRWLAECRDRNERGRITLYHGWESGMDNSPRWDSAYANVVPGKLPEYQRADNVIITDPSQRPSDGEYDRYLWLLEEMKAVRYDDERLPSVMSFQVEDVFFSAIFSVACQVLAEIGEDYKRPHADVKDLYLWAERFRAGVVETTDQRTGAARDFDVLAEKWLVTETAAQFAPLLCGGLPHDRERALLKLLEGPRFCGHPDLKYGLIPSTSPVSRDFRPREYWRGPVWPVLTWLFSWCFARRGWAERARLLRQEGLRQASDGSFAEYYEPFTGEPLGSMQQSWTAAAVLDWLG
No. of amino acids 448
Theoretical molecular weight (Da) 50897

The BsmBI recognition sequence is underlined.

The EcoRI recognition sequence is underlined.

§

The residues removed by TEV protease cleavage are italicized. The sequence of native MhGgH is underlined.