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. 2017 Sep 28;12(9):e0185418. doi: 10.1371/journal.pone.0185418

Fig 5. Expression patterns in 11 GRAS genes by real-time quantitative RT-PCR analysis.

Fig 5

cDNAs from nine different tissues of eight developmental stages were used to detect the expression of eleven GRAS genes in which ZmGRAS40, ZmGRAS54 and ZmGRAS62 have multiple splice variants. The quantitative primers for ZmGRAS40, ZmGRAS62 were used to test all of the variants, and the quantitative primers for the gene ZmGRAS54 were used to test the main two transcripts (GRMZM2G144744-T01 and GRMZM2G144744-T02). The relative expression levels which were normalized to ACTIN were determined by the comparative CT method (2−ΔΔCT ) [62] Three biological replicates were conducted for each experiment.