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The different colors represent different antibody/antigen Kd values. A) Antigen bulk concentration = 20 pM and spacer of 50 monomers. B) Antigen bulk concentration = 20 pM and no spacer. C) Antigen bulk concentration = 100 nM and spacer of 50 monomers. D) Antigen bulk concentration = 100 nM and no spacer.
