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Averaged center of mass of unbound antibodies (0 ant.), antibodies bound to one antigen (1 ant.), antibodies bound to two antigens (2 ant.) as a function of the surface coverage. A) Antigen bulk concentration = 100 nM, spacer 50 monomers, Kd = 10−9 M. B) Antigen bulk concentration = 100 nM, spacer 50 monomers, Kd = 10−11 M.
