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. 2017 Sep 28;12(9):e0185518. doi: 10.1371/journal.pone.0185518

Fig 6. Captured antigen as a function of the antibody surface coverage on AcNP conjugated through streptavidin/biotin.

Fig 6

The different colors represent different antibody-antigen Kd values. A) Bulk mix concentration = 20 pM antigen/10 pM antibody and spacer of 50 monomers. B) Bulk mix concentration = 20 pM antigen/10 pM antibody and no spacer. C) Bulk mix concentration = 200 nM antigen/100 nM antibody and spacer of 50 monomers. D) Bulk mix concentration = 200 nM antigen/100 nM antibody and no spacer.