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Amount of antibody unbound (0 ant.), antibody bound to one antigen (1 ant.), antibody bound to two antigens (2 ant.) and total antibody (total) on surface as a function of the streptavidin surface coverage. A) Bulk mix concentration = 200 nM antigen/100 nM antibody, spacer 50 monomers, Kd = 10−9 M. B) Bulk mix concentration = 200 nM antigen/100 nM antibody, spacer 50 monomers, Kd = 10−11 M.
