Table 4. Repeatability and reproducibility of the real time PCR assay.

	Intra assay variation of Ct values						Inter assay variation of Ct values
Parasite Load	Replicate 1	Replicate 2	Replicate 3	Mean	SD	CV%	Assay 1	Assay 2	Mean	SD	CV%
1x10 5	17.20	17.21	17.19	17.20	0.01	0.06	17.20	18.10	17.64	0.64	3.63
1x10 4	20.79	20.72	20.74	20.75	0.04	0.17	20.75	21.39	21.07	0.45	2.14
1x10 3	24.11	24.11	24.11	24.11	0.00	0.00	24.11	24.76	24.43	0.46	1.88
1x10 2	27.56	27.43	27.55	27.51	0.07	0.26	27.51	28.04	27.78	0.38	1.37
1x10 1	30.73	30.68	30.65	30.69	0.04	0.13	30.69	31.44	31.07	0.53	1.71
1x10 0	34.77	33.51	34.26	34.18	0.63	1.85	34.18	34.41	34.30	0.16	0.47
1x10 -1	36.59	37.56	36.51	36.89	0.34	0.93	36.89	38.46	37.67	1.11	2.95

SD, standard deviation; CV, Co-efficient of variation