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. 2017 Sep 28;12(9):e0185662. doi: 10.1371/journal.pone.0185662

Fig 2. Expression and SEC analysis of the soluble form of rbFcRn purified from Sf9 cell supernatant.

Fig 2

The expression of rbFcRn was detected by 15% SDS-PAGE followed by Coomassie staining. The rbFcRn α-chain, as well as the rbβ2m was observed around 28 and 12 kDa, respectively (Panel A). By using anti-His tag monoclonal Ab in Western blot experiments only the rbFcRn α-chain containing 6xHis-tag can be detected (Panel B). As a control, soluble hFcRn was used in both experiments. The purity of rbFcRn (Panel C) and rbIgG (Panel D), as well as hFcRn (Panel E) and hIgG1 (Panel F) samples was verified by size-exclusion chromatography.