Table 2. Kinetic parameters of the interaction of immobilized rbFcRn and hFcRn with rbIgG and hIgG isotypes at pH 6.0.
| rbFcRn | hFcRn | |||||||
|---|---|---|---|---|---|---|---|---|
| KD1 (M) x 10−9 |
f1 (%) | KD2 (M) x 10−9 |
f2 (%) | KD1 (M) x 10−9 |
f1 (%) | KD2 (M) x 10−9 |
f2 (%) | |
| rbIgG | 20.3 ± 2.21 | 68.4 | 49.3 ± 1.78 | 31.6 | 6.58 ± 0.12 | 77.0 | 15.9 ± 0.27 | 23.0 |
| hIgG1 | 12.9 ± 1.83 | 28.8 | 71.9 ± 15.7 | 71.2 | 9.99 ± 0,43 | 42.4 | 57.2 ± 3.05 | 57.6 |
| hIgG2 | 7.46 ± 1.41 | 84.3 | 106 ± 31.5 | 15.7 | 5.43 ± 0.26 | 89.2 | 34.7 ± 0.68 | 10.8 |
| hIgG3 | 2.57 ± 0.52 | 49.9 | 59.2 ± 7.25 | 50.1 | 6.06 ± 0.25 | 74.8 | 17.4 ± 0.50 | 25.2 |
| hIgG4 | 3.86 ± 0.79 | 89.8 | 53.5 ± 20.0 | 30.5 | 2.36 ± 0.14 | 92.1 | 20.2 ± 1.01 | 7.9 |
The KD values (mean ± S.E.) were calculated from the estimated association and dissociation rate constants (KD1 = kd1/ka1) and (KD2 = kd2/ka2) using heterogeneous ligand model during the fitting procedure (n = 2–3).