Fig 4. AR and its ligand specifically promote the phosphorylation of EphA2 at Ser897.

(a, b) The level of EphA2 that is phosphorylated at Ser897 is specifically upregulated upon KSHV infection, and the effect is significantly inhibited by AR and EphA2 siRNA treatment. siRNAs were transfected into cells at final concentrations of 100 nM for HUVECs and 200 nM for SLK cells using Lipofectamine 2000. siRNA-transfected cells were serum-starved for 6 h prior to the inoculation of KSHV at the indicated MOIs for the indicated times. Cells were lysed in the presence of protease and phosphatase inhibitors and subjected to western blotting. (c, d) DHT treatment specifically increases EphA2 phosphorylation at Ser897 upon KSHV infection. Cells were left untreated, or treated with DHT or ethanol at 1 or 3 uM as described previously, infected with KSHV, and lysed as described above. Each reaction was repeated in, at least, triplicate.