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. 2017 Sep 18;11(9):e0005908. doi: 10.1371/journal.pntd.0005908

Fig 1. In vitro expression of the glycoprotein genes from the CCHFV-M DNA vaccine plasmid.

Fig 1

A) The total (permeabilized cells) and surface presence (non-permeabilized cells) of GC was examined 44 h after transfection of COS-7 cells with wild-type CCHFV-M, optimized CCHFV-M, or empty vector, the maximum expression was seen at 250 ng of each plasmid (shown). B) In vitro expression by Western blot of GN (37 kDa) and GC (75 kDa) in COS-7 cells 44 h after transfection of CCHFV-M or empty vector, 250 ng of each plasmid.