Table I. Number of annotations, pseudospectra, and base peak annotations after each filter step.

Filter Step	Annotations	Pseudospectra Annotations	Base Peak Annotations
All annotations (1.5 ppm)	5,050	2,612	3,322
Lipids of interesta	3,201	2,165	2,026
Isotope filter	3,112	2,123	1,987
Bothmode filter	2,939	2,110	1,957
Adduct filter	1,079	1,014	928
Retention filter	1,066	1,003	918
MSMS filter	624	607	556
MSMS filter (+FA)b	778	761	681

^aTAG, DAG, MAG, PC, PE, PG, PI, lysoPC, lysoPE, lysoPG, lysoPI, MGDG, and DGDG.  ^bFAs are not MS/MS validated.