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. 2017 Aug 22;175(2):848–857. doi: 10.1104/pp.17.00232

Figure 5.

Figure 5.

BBM-induced embryogenesis is context dependent. A, 35S:BBM-GR plants were cultured with 10 µm DEX starting at the dry seed stage (d0) or after germination (d4). The culture time after DEX application is indicated on the bottom right of each image. Arrowheads, Callus; arrows, somatic embryos/embryogenic tissue; le, callused leaf tissue. The bottom image is a magnification of the boxed region in the d4 +14 image. B, LEC1:LEC1-GFP regulation by BBM. Seedlings were treated with 10 µm DEX after germination (d4) and imaged on subsequent days as indicated. The images show the adaxial sides of cotyledons. Arrowhead, Callus on the distal end of the cotyledon blade; arrows, GFP-positive embryo clusters. The outline of the cotyledon margins is shown with dashed lines. Autofluorescence (magenta) was used to delineate the tissue. Bars = 250 μm.