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. 2017 Aug 18;175(2):816–827. doi: 10.1104/pp.17.00814

Figure 6.

Figure 6.

Lineage inheritance in the early stage of pattern formation is modified by positional information. A and B, Simultaneously imaging the expression of pWER:H2B-mCherry (pWER:HC) and pCO2:HY in roots of wild-type (A) and pWER:SHR-expressing line (B). Yellow arrowheads point to cells losing pWER:HC expression and white arrow head marks the starting cell that adopts the expression of pCO2:HY. C and D, Lignified Casparian strips visible as green autofluorescence of cell walls after clearing in pWER:SHR-expressing roots (marked by the white arrows). Double-headed arrows represent cell layers outside of endodermis. E, PI penetrated into all cell layers outside of endodermis (marked by the double-headed arrow). White arrowhead points to the position of endodermis where PI penetration was blocked. F, pPIN2:PIN2-GFP in the root expressing 35S:SHR. Ep, epidermis; Ex, extra cell layers. Red lines depict the basal side of PIN2-GFP in extra cell layers, and yellow lines depict the apical side of PIN2-GFP in epidermis. Scale bars = 20 μm.