Table I. Kinetic parameters of full-length and truncated BnaDGAT1 using palmitoyl-CoA and oleoyl-CoA as acyl donors.
The data for the substrate saturation assays were fitted with the Hill equation using GraphPad Prism 7.0a. The apparent Vmax values were not normalized for DGAT abundance. Reported values are means ± sd; n = 3.
| Parameter | BnaDGAT11-501 |
BnaDGAT181-501 |
||
|---|---|---|---|---|
| Oleoyl-CoA | Palmitoyl-CoA | Oleoyl-CoA | Palmitoyl-CoA | |
| Apparent Vmax (nmol TAG min−1 mg−1 protein) | 5.58 ± 0.14 | 5.75 ± 0.16 | 2.27 ± 0.24 | 2.18 ± 0.09 |
| Hill coefficient | 2.28 ± 0.13 | 2.65 ± 0.19 | 1.26 ± 0.19 | 1.64 ± 0.11 |
| Apparent S0.5 (μm) | 0.61 ± 0.02 | 0.69 ± 0.02 | 0.55 ± 0.11 | 0.67 ± 0.05 |
| Goodness of fit/R2 | 0.994 | 0.992 | 0.961 | 0.992 |