Table II. Kinetic parameters of BnaDGAT1 using oleoyl-CoA as an acyl donor in the presence of varying amounts of CoA.

The data for the substrate saturation assays were fitted with the Hill equation using GraphPad Prism 7.0a. Reported values are means ± sd; n = 3. CoA was found to be a noncompetitive inhibitor of BnaDGAT1.

[CoA]	Apparent Vmax	Apparent S0.5
	nmol TAG min−1 mg−1 protein	μm
Control	5.55 ± 0.17	0.61 ± 0.02
10 μm	4.56 ± 0.25	0.60 ± 0.04
50 μm	2.46 ± 0.16	0.62 ± 0.05