Figure 2.
Secretion is inhibited in chc1 mutants. A, The expression of a multicistronic vector expressing secYFP, GFP-HDEL, and the cytoplasmic fragment of the SNARE protein SYP121 (SYP121ΔC) inhibits secYFP secretion (top row) compared with the empty vector (bottom row). SYP121ΔC blocks secretion in wild-type (Col-0) roots and enhances secretory blockage in the chc1 mutants. Bars = 50 μm. B, Graphical representation of the fluorescence data presented in A. Letters indicate statistical significance by ANOVA, where P < 0.005 using the Holm-Sidak model. Values are means ± se; n > 6 seedlings per genotype. Representative data from one of at least three independent experiments are shown.