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. 2017 Sep 13;6:e29257. doi: 10.7554/eLife.29257

Figure 4. Structure-function analysis for CASY-1 and RIG-6.

Figure 4.

(A and C) Domain structure of mRFP::CASY-1 and its deletion constructs (A) or of YFP::RIG-6 and its deletion constructs (C) with the summary of rescue activity when expressed in AVG of casy-1 or rig-6 mutant animals. SP, signal peptide; Cad, cadherin domain; LNS, laminin neurexin sex hormone binding protein domain; TM, transmembrane; Intra, intracellular domain; Ig, immunoglobulin domain; FN[III], fibronectin type III domain. (B and D) Percentage of axon-axon contact between each neuronal pair in animals expressing the indicated deletion constructs for CASY-1 (B) or RIG-6 (D) in AVG of casy-1 or rig-6 mutant animals (n = 30). Note that Full-Ct (CASY-1::YFP) in (B) and Full (YFP::RIG-6) in (D) were the same transgenes used for protein localization in Figure 3, and rescued axon fasciculation defects in mutants. The data for wild type and casy-1 and rig-6 mutants are the same as shown in Figure 2. Each dot represents individual animal. Red bar represents the median. *p<0.05; **p<0.01; ***p<0.001; ns, not significant (by Mann-Whitney test). For the data and statistics, see Figure 4—source data 1.

Figure 4—source data 1. Source data for Figure 4.
elife-29257-fig4-data1.xlsx (27.3KB, xlsx)
DOI: 10.7554/eLife.29257.014