A fosmid-based reporter line carrying
sax-7::gfp was crossed with animals carrying
eat-4fosmid::mCherry or
unc-47fosmid::mCherry and examined for expression in PHC, HOA or AVG in L4 or adult males. Note that SAX-7 is localized to the cytoplasm and plasma membrane in neuronal cell bodies, while mCherry-tagged proteins are targeted to the nucleus due to the nuclear localization signal of H2B. Location of PHC, HOA or AVG cell body is indicated as an arrow. Identities of
eat-4 or
unc-47 expressing cells in males are previously described (
Gendrel et al., 2016;
Serrano-Saiz et al., 2017b). SAX-7 was expressed in PHC both in L4 and adult males, but in the L4 stage it was easier to detect the signal than in the adult stage where SAX-7 expression in male-specific ray neurons near PHC was more intense. The image for
unc-47fosmid::mCherry was overexposed to show AVG expression. Scale bars, 10 μm.