Figure 2.

Dietary calorie intake reduction prevents growth-induced glomerular failure in the AA-4E-BP1 model in a dose-dependent manner. Dietary calorie intake reduction (40% reduction>20% reduction) has the following protective effects: body weight gain was slowed (A); kidney weight gain was reduced (F); glomerular tuft volume was reduced (I); rate of podocyte detachment was reduced (D); reduction of podocyte number per glomerular tuft was prevented (K); reduction in podocyte density was prevented (L); the increase in podocyte cell volume was prevented (J); urine protein/creatinine ratio was reduced (C); percentage of glomeruli with adhesions and amount of glomerulosclerosis were reduced (G and E1, 2, and 3); decreased creatinine clearance was prevented (H); and systolic BP increase with age was prevented (B). Data shown as the mean±SEM. Glomerular tuft volume (μm³), mean podocyte volume (μm³), podocytes per tuft (n) and podocyte nuclear density (n per 106 μm³). *P<0.05, **P<0.01. Ccr, creatinine clearance; Cre, urine creatinine; glom, glomerulus; W, weeks.