Table 4.
Ingenuity Pathway Transcript Analysis
| IPA Rank Order | Categories | Diseases or Functions Annotation | P Value | Molecules | Molecule Number |
|---|---|---|---|---|---|
| 1 | Cell cycle, | Segregation of | 7.4E-25 | ATRX,AURKB,BUB1,CCNA2,CCNB1,CCNB2,CDC14A, | 34 |
| cellular assembly | chromosomes | CENPE,CENPF,CENPT,ECT2,HJURP,HMMR,KIF11,KIF2C, | |||
| and organization, | KMT5B,KNSTRN,LATS1,LMNA,NCAPD2,NCAPD3,NCAPG, | ||||
| DNA replication, | NDC80,NINL,NUF2,NUSAP1,PLK1,PTTG1,SGO1,STAG1, | ||||
| recombination, | STAG2,TOP2A,TPX2,ZWINT | ||||
| and repair | |||||
| 2 | Cell cycle | Arrest in mitosis | 1.6E-17 | AURKB,BUB1,BUB1B,CDC20,CDK1,CENPE,CENPI,CSNK1A1, | 23 |
| DCTN2,KIF11,KIF4A,KNL1,KNTC1,MYBL2,NDC80,NUF2, | |||||
| PDLIM7,PLK1,PURA,RACGAP1,SGO1,TPX2,ZWINT | |||||
| 3 | Cell cycle | Mitosis | 1.9E-17 | AURKB,BCL2,BMP2,BUB1,BUB1B,CCNA2,CCNB1,CDC14A, | 57 |
| CDC20,CDC25C,CDK1,CENPE,CENPF,CENPI,CENPT, | |||||
| CLASP2,CSNK1A1,DCTN2,DLGAP5,DYNLT3,FBXW5,FOXM1, | |||||
| GADD45B,JUN,KIF11,KIF18A,KIF18B,KIF2C,KIF4A,KNL1, | |||||
| KNTC1,LATS1,LATS2,MYBL2,NDC80,NINL,NRG1,NUF2, | |||||
| NUSAP1,PDGFRA,PDLIM7,PHB2,PHIP,PLK1,PTHLH,PTTG1, | |||||
| PURA,RACGAP1,SGO1,SLC9A3R1,SPP1,TNC,TOP2A,TPX2, | |||||
| TRIM33,YWHAE,ZWINT | |||||
| 4 | Cell cycle | M phase | 1.1E-16 | AURKB,BCL2,BUB1B,CCNB1,CD2AP,CDC14A,CDC20,CDK1, | 37 |
| CENPE,DIAPH3,ECT2,GIPC1,HMMR,KIF14,KIF20A, | |||||
| KIF20B,KIF23,KIF4A,KLHL9,LATS1,LATS2,LMNA,mir-23, | |||||
| NCAPD2,NDC80,NUF2,NUSAP1,PFN1,PLK1,PRC1,PTTG1, | |||||
| RAB11FIP3,RACGAP1,RHOC,STAG1,TOP2A,TOPAZ1 |
Ingenuity Pathway Analysis of glomerular genes. Five hundred thirty-eight genes (shown in Supplemental Table 2) were expressed significantly differently (either up or down) between the TG.1K.ALD rats that developed FSGS and the three groups that were prevented by treatment from developing FSGS (TG.1K.CRD, TG.1K.Rapa, and TG.1K.ACEi). Ingenuity Pathway Analysis of these genes in the Diseases or Functions annotation showed that the top 35 were designated either as cell cycle or various cancers. The top four candidates, all designated as different aspects of cell cycle, with overlapping genes, are shown. Transcript levels for the 84 genes identified in Table 5 relative to the TG.1K.ALD group are shown in Supplemental Table 3. IPA, Ingenuity Pathway Analysis.