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. Author manuscript; available in PMC: 2017 Sep 28.
Published in final edited form as: Sci Signal. 2016 Dec 6;9(457):ra118. doi: 10.1126/scisignal.aaf5402

Figure 3. TGFβ1 depletion skews lineage commitment in mammospheres.

Figure 3

(A and B) Representative images of immunostaining for (A) p63 (red) and Ki-67 (green) or (B) K14 (red) and K18 (green) in WT and Tgfb1 HT mammospheres, treated as indicated. Scale bars, 100 μm.(C)Frequency of p63+ cells in Tgfb1 HT or WT primary mammospheres. (D) Frequency of p63+ cells in WT secondary mammospheres treated with LY364947 in culture compared to vehicle control. (E) Frequency of p63+ cells in WT mammospheres treated with 1D11 or antibody control. Scale bar, 100 μm. Data in (C-E) are means ± S.E.M. from three biological replicates; *P<0.05, **P<0.01, by Student's t-test.