Fig. 5.

Impact of NAC on diabetes-induced alterations in GSH and MG: HPLC was used to measure GSH, MG or GSSG (A-F) and western blot was used to probe for MG-adducts (G) in platelets isolated from mice 6 or 20 wks after injection with vehicle (Veh), or streptozotocin to induce diabetes (STZ). Separate STZ groups were treated with N-acetylcysteine in drinking water for 3 wks before obtaining platelets (STZ +NAC). In (G), a sample blot and the quantification of 24kD and 54kD protein band intensities normalized to β-actin are shown. * P < 0.01 vs. Veh; # P < 0.01 vs. STZ.