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. 2017 Sep 20;14:218–228. doi: 10.1016/j.redox.2017.09.005

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© 2017 Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. S1 — Examples of flow cytometry analysis for platelet-leukocyte aggregates (PLAs). Left: CD45 (leukocytes), CD41 (platelets) double positive events identified platelet-leukocyte aggregates. Right: This population was further divided into F4/80+ (Pl-Mono aggregates), Ly-6 G+ (Pl-Neut aggregates) or F4/80-negative, Ly-6 G-negative (Pl-Lymph aggregates). Examples are provided from the three experimental groups: mice injected with vehicle (Veh 19 week), mice injected with STZ to induce diabetes (STZ 19 week) and STZ-treated mice given NAC in drinking water for 2 wks before observation (STZ 19 week+NAC 2wk).