Figure 6. c-Met inhibitors suppressed tumor-sphere formation of ALDH1^high breast cancer cells.

A. Tumor-spheres of ALDH1^high cells derived from MDA-MB157 cells were incubated with c-Met inhibitors, Crizotinib (1 μM), Foretinib (1 μM), PHA-665752 (10 μM) and Tivantinib (1 μM) for 6 days. B. ATP level was assessed by the Cell-Titer Glo assay after treating tumor-spheres for 6 days with c-Met inhibitors, Crizotinib, Foretinib, Tivantinib (0.03, 0.1, 0.3, 1, and 3 μM) and PHA-665752 (0.6125, 1.25, 2.5, 5, and 10 μM). C. In vitro IC₅₀ values with respect to decrease in ATP level on treatment with c-Met inhibitors. Numerical values of test groups are shown with respect to 0.02% DMSO treated group. All data is represented as the mean± S.D. from three independent experiments. Scale bar, 100 μm.