Table 1.

Data collection, refinement, and validation statistics

Data collection
Microscope	Titan KRIOS with Cs-corrector
Camera	Falcon 2
Voltage	300 kV
Pixel size (Å)	1.105
Total dose (e−Å−2)	25
Micrographs collected (#)	5275
Refinement
Total number of particles (#)	163,121
Particles in 50S masked map (#)	43,530
Resolution (Å; at FSCa = 0.143)	5.6
CCa (model to map fitb)	0.800
RMS a deviations
Bonds (Å)	0.008
Angles (°)	1.262
Chirality (°)	0.06
Planarity (°)	0.008
Validation c
Clash-scored	18.69
Proteins
MolProbity score	2.55
Favored rotamers	91.3%
Ramachandran favored	82.31%
Ramachandran allowed	17.54%
Ramachandran outliers	0.15%
RNA
Correct sugar puckers	99.49%

^aFourier shell correlation; correlation coefficient; root-mean square

^bOnly across atoms in the model; as indicated in Phenix⁴⁷

^cAs indicated by MolProbity⁵²

^dNumber of steric overlaps (>0.4 Å) per 1000 atoms