Fig. 2.

MRAP2 is important for the stimulation of AGRP neurons by fasting. a–d cFos expression in the arcuate nucleus of fed WT (a), 24 h-fasted WT (b), fed Mrap2 KO (c), and 24 h-fasted Mrap2 KO (d) mice. e Quantitative measurement of cFos activation in the arcuate nucleus of fed or fasted WT and Mrap2 KO mice. f, g NPY expression measured by qPCR in the arcuate nucleus of male (f) and female (g) fed or 24 h-fasted WT and Mrap2 KO mice. h, i AGRP expression measured by qPCR in the arcuate nucleus of male (h) and female (i) fed or 24 h-fasted WT and Mrap2 KO mice. j POMC expression measured by qPCR in the arcuate nucleus of male fed or 24 h-fasted WT and Mrap2 KO mice. k–m Colocalization of AGRP (k) and MRAP2 (l) by immunofluorescence microscopy in the arcuate nucleus. AGRP neurons expressing MRAP2 are shown in yellow in the merged image (m). Error bars are SEM. ***p < 0.001, **p < 0.01