Fig. 3.

MRAP2 is important within AGRP neurons for starvation sensing. a qPCR measurement of MRAP2 expression in the ARC of AGRP^CRE/MRAP2^+/+ and AGRP^CRE/MRAP2^fl/fl mice. b Food intake after a 24 h fast in AGRP^CRE/MRAP2^+/+ and AGRP^CRE/MRAP2^fl/fl mice (n = 8 AGRP^CRE/MRAP2^+/+, n = 5 AGRP^CRE/MRAP2^fl/fl). c–f Immunofluorescent detection of cFos expression in the arcuate nucleus of fed or fasted AGRP^CRE/MRAP2^+/+ and AGRP^CRE/MRAP2^fl/fl mice. g Quantification of fasting-induced cFos expression in the arcuate nucleus of AGRP^CRE/MRAP2^+/+ and AGRP^CRE/MRAP2^fl/fl mice (n = 3/group). Error bars are SEM. ***p<0.001, **p < 0.01, *p<0.05