Figure 3. DAB2IP epigenetically regulates miR-138 expression.

(A) Expression levels of primary, precursor, and mature miR-138 were compared in 786O and HK-2 sublines (Con, KD). (B and C) 786O KD (B) and 769P (C) cells were transfected with increasing dosage of DAB2IP for 48 h, and relative expressions of primary and precursor miR-138 were analyzed by qRT-PCR. (D) Cells were treated with 5-Aza (5 μM) and TSA (100 nM) for 72 h, and pri-miR138-1 expressions were analyzed.