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. 2017 May 9;8(40):66975–66986. doi: 10.18632/oncotarget.17756

Figure 5. miR-138 targets ABCA13 and EZH2 mRNA via the 3′-UTR regions.

Figure 5

(A–C) Expression levels of ABCA13 and EZH2 in RCC cells were analyzed by qRT-PCR (A), or immunofluorescence staining (B), or Western blot analysis (C). (D) Luciferase reporter activities in 786O cells transfected with either ABCA13 or EZH2 3′UTR reporter construct for 48 h. The luciferase reporter activity was measured with the dual luciferase assay. (E) The inhibitory effect of miR-138 on 3′UTR reporter activities or mRNA expression of ABCA13 and EZH2 in HK-2 KD cells were analyzed. (F and G) The protein expressions of ABCA13 (F) and EZH2 (G) were determined in HK-2 KD transfected with miR-138 expression vector.