Figure 6. EZH2 plays a critical role in maintaining stem-cell like properties of renal cells.

(A) Cells were transfected with EZH2 expression plasmid for 48 h then expression of DAB2IP and miR-138 levels were determined by qRT-PCR. (B) Cells were treated with EZH2 inhibitor GSK126 (1 μM) for 48 h, then DAB2IP and miR-138 expressions were determined. (C–E) Cells were transfected with miR-138 and/or EZH2 expression vector as indicated, the sphere forming ability (C), colony forming ability (D), or SP (E) was determined separately.