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. 2017 Apr 26;8(40):67001–67016. doi: 10.18632/oncotarget.17433

Figure 1. Elevated dicer protein levels are linked to a thermotolerant phenotype in HeLa and murine embryonic fibroblast (MEF) cell lines.

Figure 1

(A) Western blot analysis of dicer, HSP70 and GAPDH protein levels in HeLa cells at 39.5°C for 0h, 6h and 16h. Experiments were conducted under untransfected (UT), control-transfected (Ctrl siRNA) or dicer knockdown (dicer siRNA) conditions. (B) Protein band intensities in (A) were first normalized to the GAPDH band intensities corresponding to the specific protein and further normalized to the 0h time point in the untransfected group within each biological replicate. (C) Western blot analysis of experiments in MEF cells conducted as described in (A). (D) Band intensities in (C) analyzed as described in (B). (E) MTT cell viability assays in control (37°C) HeLa cells heated at 43°C (C); mild hyperthermia (6h at 39.5°C) pretreated (PT) cells subsequently heated at 43°C (6h PT); and mild hyperthermia (16h at 39.5°C) pretreated cells subsequently heated at 43°C (16h PT). (F) MTT cell viability assays in MEF cells conducted as described in (E). The representative western blot images for GAPDH correspond to the western blot image for dicer in this figure. Values shown are mean ± SEM. n = 3, *p < 0.05.