Figure 2. Elevated dicer protein levels observed during mild (39.5°C) hyperthermia-induced thermotolerance are not linked to HSP70 protein levels.

(A) Western blot analysis of HSP70 protein levels in wildtype (WT) and dicer deficient (DCR−/−) HCT116 cells at mild hyperthermia (39.5°C) for 16h. (B) Protein band intensities in (A) were first normalized to the actin band intensities corresponding to the specific protein and further normalized to the first experimental replicate in control (lane 1) and mild hyperthermia (lane 7) samples within each biological replicate. The representative western blot images for Actin correspond to the western blot image for dicer in this figure. Values shown are mean ± SEM. p < 0.05.