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. 2017 Apr 26;8(40):67001–67016. doi: 10.18632/oncotarget.17433

Figure 4. Mild hyperthermia (39.5°C) stress-induced thermotolerance does not significantly alter dicer activity in HeLa cells.

Figure 4

(A) Urea PAGE analysis of radiolabeled pre-microRNA-124a and mature microRNA-124a in HeLa cells at 39.5°C for 0h, 6h and 16h. Gel lanes excluded from analysis are crossed out with broken lines. (B) The ratio of the band intensities of the mature microRNA to that of the pre-microRNA (i.e. relative 22nt mature MicroRNA band intensity) in lanes containing cell lysate alone, or cell lysate supplemented with RNase inhibitor. (C) Relative band intensities of lanes containing lysates from dicer LOF and wildtype (WT) MEFs. (D) Relative band intensities of lanes containing 10, 20 and 30 ug of total protein from lysate alone, or lysate supplemented with RNase inhibitor. Lanes excluded from the analysis are marked with broken lines. Values shown are mean ± SEM. n = 3; *p < 0.05.