Skip to main content
. 2017 Apr 26;8(40):67001–67016. doi: 10.18632/oncotarget.17433

Figure 6. Elevated dicer protein levels observed during mild (39.5°C) hyperthermia-induced thermotolerance influence ATF4 and CHOP protein levels in HeLa and MEF cells.

Figure 6

(A) Western blot analysis of dicer, ATF4, CHOP and GAPDH protein levels in HeLa cells at 39.5°C for 0h, 6h and 16h under untransfected (UT), control-transfected (Ctrl siRNA), and dicer knockdown (dicer siRNA) conditions. (B) ATF4 band intensities in (A) were first normalized to GAPDH band intensities corresponding to ATF4 (not shown) and further normalized to the 0h value in the untransfected group. (C) CHOP band intensities in (A) were first normalized to GAPDH band intensities corresponding to CHOP (not shown) and further normalized to the 0h value in the untransfected group. (D) Western blot analysis as described in (A) conducted in MEF cells (E). ATF4 band intensity analysis as described in (B) conducted in MEF cells (F). CHOP band intensity analysis as described in (C) conducted in MEF cells. The representative western blot images for GAPDH correspond to the western blot image for dicer this figure. Values shown are mean ± SEM. n = 3, *p < 0.05.