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. 2017 May 15;8(40):67082–67093. doi: 10.18632/oncotarget.17859

Figure 1. HKST protected cells against radiation-induced cell death and DNA damage.

Figure 1

HUVEC cells were pretreated with HKST (107/mL) for 12h and exposure to IR. Cell apoptosis were determined at 24h post irradiation by flow cytometry (A). Annexin V positive cells were quantified in different groups (B). HUVEC cells (C) and L02 cells (D) were exposed to 0, 2, 4, 8Gy irradiation and colony formation assay were used to measure cell survival. A γ-H2AX foci assay was used to determine DNA damage in HUVEC cells at 0, 0.5, 8, 24h after exposure to 3Gy irradiation (E). The numbers of γ-H2AX foci per cell in different groups were counted (F). (n=6) *P<0.05, **P<0.01 Vs IR groups.