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. 2017 May 22;8(40):67169–67180. doi: 10.18632/oncotarget.18052

Figure 3. MiR-199a/b-5p overexpression reduces HCC proliferation and metastasis.

Figure 3

(A) The relative expression of miR-199a/b-5p in SMMC-7721 and HepG2 cells transfected with 80nM miR-199a/b-5p mimics (1:1) or control. (B) Estimation of cell proliferation in SMMC-7721 and HepG2 cell lines transfected with control or miR-199a/b-5p mimics by cell counting kit-8 (CCK-8) assays. (C) Transwell assays determining cell migration and invasion potential of SMMC-7721 and HepG2 cell lines transfected with control or miR-199a/b-5p mimics. (D) In vivo xenograft tumor nude mice model determining the effects of miR199a/b-5p overexpression on HCC metastasis and mice survival. SMMC-7721 cells with control or stably overexpressed miR-199a/b-5p were injected into the caudal vein of mice and examined 14 days after cells implantation at the In Vivo Imaging System (IVIS). Kaplan-Meier survival curves of mice implanted with control or stably overexpressed miR-199a/b-5p SMMC-7721 cells are shown. The results are expressed as mean ± SD (*p<0.05, **p<0.01).