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. 2017 May 22;8(40):67169–67180. doi: 10.18632/oncotarget.18052

Figure 5. ROCK1 regulates HCC migration, and invasion.

Figure 5

(A) ROCK1 mRNA expression levels in 50 normal liver and 374 HCC tumor samples from the TCGA RNA-Seq database. (B) qRT-PCR analysis of ROCK1 mRNA levels in 35 HCC and matched normal liver samples. (C) qRT-PCR analysis of ROCK1 mRNA levels in immortalized normal hepatic cell line, QSG-7701 and HCC cell lines, SMMC-7721 and HepG2. (D) Western blot analysis of ROCK1 protein in immortalized normal hepatic cell line, QSG-7701 and HCC cell lines, SMMC-7721 and HepG2. (E) Transwell assays showing cell migration and invasion potential of control and ROCK1 siRNA transfected SMMC-7721 and HepG2 cell lines. (F) qRT-PCR analysis of miR-199a/b-5p expression in ROCK1 knockdown SMMC-7721 cells transfected with miR-NC or miR-199a/b-5p. (G) In vivo xenograft tumor nude mice model determining the effects of ROCK1 knockdown on HCC metastasis. Control and ROCK1 siRNA transfected SMMC-7721 cells were injected into the caudal vein of mice and examined after 14 days at the In Vivo Imaging System (IVIS). Kaplan-Meier survival curves of mice implanted with control and ROCK1 siRNA transfected SMMC-7721 cells are shown. The results are expressed as mean ± SD (*p<0.05, **p<0.01, n.s means no significance).