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. 2017 Jun 5;8(40):67218–67226. doi: 10.18632/oncotarget.18365

Figure 6. Hernandezine inhibits LPS-induced TNFα production in primary human PBMCs.

Figure 6

Ex-vivo cultured PBMCs of COPD patients were treated with LPS (100 ng/mL) and/or hernandezine (30 μM) for indicated time, cell survival was tested by trypan blue assay (A); relative TNFα mRNA expression ((B), qRT-PCR assay) and TNFα content in conditional medium ((C), ELISA assay) were also tested. * p < 0.05 vs. “C” group. # p < 0.05 vs. LPS only group. Experiments in this figure were repeated three times, and similar results were obtained.