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. 2017 Jun 21;8(40):67254–67268. doi: 10.18632/oncotarget.18595

Figure 3. Hyperthermia-increased P2X7 functionality is independent of lipid rafts.

Figure 3

(A-B) MCA38 cells were treated with methyl β-cyclodextrin (MCD) (left) or filipin (right) (A) or cholesterol (B), before being pulse treated with ATP (1 mM) for 15 min at 40°C. 24 hr later, cell viability was evaluated. Cells treated with media served as control (Ctrl). (C) Cholesterol staining with filipin showing cholesterol rearrangement at the plasma membrane after treatment with hyperthermia and ATP. *p < 0.05 in contrast with control (two-way ANOVA, followed by Bonferroni pos-test, mean ± SD). Bars, 20 μM.