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. 2017 Jun 21;8(40):67254–67268. doi: 10.18632/oncotarget.18595

Figure 4. The membrane fluidizer benzyl alcohol (BA) acts similarly as hyperthermia leading to P2X7 hyperactivation at 37°C.

Figure 4

NC (negative control) or P2X7 KD (P2X7-deficient) cells were exposed to BA alone or together with ATP for 15 min at 37°C (in order to mimic the heat effect per se) or 40°C. Cells treated with media served as control (Ctrl). (A) Cell viability and (B) images of live cells (upper panel) and FACS analyses of NC cells (middle and bottom panels) were determined. (C) Western blot analysis of AKT/PRAS40/mTOR pathway components was performed immediately after treatment. (D) Re-organization of cholesterol-rich microdomains in co-treated NC cells was visualized using filipin. *p < 0.05 when compared to control (two-way ANOVA, followed by Bonferroni pos-test, mean ± SD). Bars, 50 μM (B) and 20 μM (D).