Skip to main content
. 2017 Jun 27;8(40):67287–67299. doi: 10.18632/oncotarget.18617

Figure 3. MYSM1 is regulated by UV-exposure of the skin and by growth factor signals in A375 melanoma cells.

Figure 3

(A) Schematic illustration of the UVB-irradiation protocol used to induce DNA-lesions in Mysm1−/− mice and WT littermates. (B) Mysm1 expression in mouse skin exposed to UVB over the course of 4 weeks compared with aged-matched untreated skin (Mysm1 red). (C) γH2AX foci in basal cells of UVB-treated WT and Mysm1−/− KO mice (γH2AX green, n≥3). For the corresponding quantification of Mysm1- and γH2AX-positive cells, positive cells in the basal cell layer in at least 15 high power fields were counted (n>3). (D) Western Blot analysis of MYSM1 protein expression in A375 melanoma cells in culture in response to serum-starvation and treatment with either MEK inhibitor PD184,352 or Akt1/2 kinase inhibitor A6730 (2 μM, both Sigma Aldrich) or both for 24 hrs compared to Δ-Actin.